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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
06/06/2019 |
Actualizado : |
06/06/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BESIL, N.; CESIO, V.; LUQUE, E.; PINTOS, P.; RIVAS, F.; HEINZEN, H. |
Afiliación : |
NATALIA BESIL, Universidad de la República (UdelaR)/ Facultad de Química; VERÓNICA CESIO, Universidad de la República (UdelaR)/ Facultad de Química; MAYZA ELEANA LUQUE NUÑEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PEDRO MAXIMILIANO PINTOS RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HORACIO HEINZEN, Universidad de la República (UdelaR)/ Facultad de Química. |
Título : |
Dissipation of pre-harvest pesticides on "Clementine" mandarins after open field application, and their persistence when stored under conventional postharvest conditions. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Horticulturae. 2018, vol.4, Issue 4:55. |
DOI : |
10.3390/horticulturae4040055 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 13 October 2018 / Revised: 11 December 2018 / Accepted: 12 December 2018 / Published: 18 December 2018.
This article belongs to the Special Issue Postharvest Disease Development: Pre and/or Postharvest Practices. |
Contenido : |
ABSTRACT.
The dissipation of field-applied difenoconazole, imidacloprid, pyraclostrobin and spinosad on Clementine mandarins (Citrus clementina Hort. ex Tan.) under controlled conditions throughout the citrus production chain was assessed. At harvest, 42 days after application, the dissipation of these pesticides were 80, 92, and 48% for difenoconazole, imidacloprid, pyraclostrobin, respectively, and spinosad was below the level of detectability. At day 28 after application, spinosad was no longer detected. The model equations that best describe the dissipation curves of these pesticides on Clementine mandarins showed different patterns. Their half-life on Clementine, calculated by the best-fitted experimental data, were 19.2 day (1st-order model) for difenoconazole, 4.1 day (Root Factor (RF) 1st-order model) for imidacloprid, 39.8 day (2nd-order model) for pyraclostrobin and 5.8 day (1st-order model) for spinosad. These results are the first record of pyraclostrobin persistence on mandarins, showing a longer half-life in this matrix than those reported for any other fruit. The treated fruit were harvested and submitted to the usual postharvest treatments: first, a hypochlorite drenching was performed; as a second step, imazalil and wax were applied, and then the mandarins were stored at 4 °C. After 32 days, cold storage caused no significant effects on the residue levels of the four pesticides compared with those determined on freshly harvested mandarins. All residues were below their Codex and European Union (EU) maximum residue limit (MRL) for mandarin since the spray application day. MenosABSTRACT.
The dissipation of field-applied difenoconazole, imidacloprid, pyraclostrobin and spinosad on Clementine mandarins (Citrus clementina Hort. ex Tan.) under controlled conditions throughout the citrus production chain was assessed. At harvest, 42 days after application, the dissipation of these pesticides were 80, 92, and 48% for difenoconazole, imidacloprid, pyraclostrobin, respectively, and spinosad was below the level of detectability. At day 28 after application, spinosad was no longer detected. The model equations that best describe the dissipation curves of these pesticides on Clementine mandarins showed different patterns. Their half-life on Clementine, calculated by the best-fitted experimental data, were 19.2 day (1st-order model) for difenoconazole, 4.1 day (Root Factor (RF) 1st-order model) for imidacloprid, 39.8 day (2nd-order model) for pyraclostrobin and 5.8 day (1st-order model) for spinosad. These results are the first record of pyraclostrobin persistence on mandarins, showing a longer half-life in this matrix than those reported for any other fruit. The treated fruit were harvested and submitted to the usual postharvest treatments: first, a hypochlorite drenching was performed; as a second step, imazalil and wax were applied, and then the mandarins were stored at 4 °C. After 32 days, cold storage caused no significant effects on the residue levels of the four pesticides compared with those determined on freshly harvested mandarins. All residues were ... Presentar Todo |
Palabras claves : |
DEGRADATION DYNAMIC; LC-MS/MS; PESTICIDE RESIDUES. |
Thesagro : |
CITRUS. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12791/1/horticulturae-04-00055.pdf
https://www.mdpi.com/2311-7524/4/4/55
|
Marc : |
LEADER 02625naa a2200253 a 4500 001 1059829 005 2019-06-06 008 2018 bl uuuu u00u1 u #d 024 7 $a10.3390/horticulturae4040055$2DOI 100 1 $aBESIL, N. 245 $aDissipation of pre-harvest pesticides on "Clementine" mandarins after open field application, and their persistence when stored under conventional postharvest conditions.$h[electronic resource] 260 $c2018 500 $aArticle history: Received: 13 October 2018 / Revised: 11 December 2018 / Accepted: 12 December 2018 / Published: 18 December 2018. This article belongs to the Special Issue Postharvest Disease Development: Pre and/or Postharvest Practices. 520 $aABSTRACT. The dissipation of field-applied difenoconazole, imidacloprid, pyraclostrobin and spinosad on Clementine mandarins (Citrus clementina Hort. ex Tan.) under controlled conditions throughout the citrus production chain was assessed. At harvest, 42 days after application, the dissipation of these pesticides were 80, 92, and 48% for difenoconazole, imidacloprid, pyraclostrobin, respectively, and spinosad was below the level of detectability. At day 28 after application, spinosad was no longer detected. The model equations that best describe the dissipation curves of these pesticides on Clementine mandarins showed different patterns. Their half-life on Clementine, calculated by the best-fitted experimental data, were 19.2 day (1st-order model) for difenoconazole, 4.1 day (Root Factor (RF) 1st-order model) for imidacloprid, 39.8 day (2nd-order model) for pyraclostrobin and 5.8 day (1st-order model) for spinosad. These results are the first record of pyraclostrobin persistence on mandarins, showing a longer half-life in this matrix than those reported for any other fruit. The treated fruit were harvested and submitted to the usual postharvest treatments: first, a hypochlorite drenching was performed; as a second step, imazalil and wax were applied, and then the mandarins were stored at 4 °C. After 32 days, cold storage caused no significant effects on the residue levels of the four pesticides compared with those determined on freshly harvested mandarins. All residues were below their Codex and European Union (EU) maximum residue limit (MRL) for mandarin since the spray application day. 650 $aCITRUS 653 $aDEGRADATION DYNAMIC 653 $aLC-MS/MS 653 $aPESTICIDE RESIDUES 700 1 $aCESIO, V. 700 1 $aLUQUE, E. 700 1 $aPINTOS, P. 700 1 $aRIVAS, F. 700 1 $aHEINZEN, H. 773 $tHorticulturae. 2018, vol.4, Issue 4:55.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
29/09/2014 |
Actualizado : |
24/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
B - 1 |
Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
ISSN : |
0717-3458 |
DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
Thesagro : |
CITRUS; CYDIA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02405naa a2200241 a 4500 001 1050705 005 2021-06-24 008 2012 bl uuuu u00u1 u #d 022 $a0717-3458 024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI 100 1 $aFEDERICI, M. 245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource] 260 $c2012 520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. 650 $aCITRUS 650 $aCYDIA 700 1 $aMARCONDES, J.A. 700 1 $aPICCHI, S.C. 700 1 $aSTUCHI, E.S. 700 1 $aFADEL, A.L. 700 1 $aLAIA, M.L. 700 1 $aLEMOS, M.V.F. 773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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